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Predicting Gene Silencing Through the Spatiotemporal Control of siRNA Release from Photo-responsive Polymeric Nanocarriers
Published on: July 21, 2017
Development of photoresponsive RNA towards photoswitching of RNA functions
Hiroshi Itoh1, Hidenori Nishioka, Xingguo Liang
1Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
Abstract:
By introducing azobenzene into RNA via a D-threoninol linker, the photoresponsive RNA was developed for the photoswitching of RNA hybridization. The T(m) measurements showed that RNA/RNA duplex formation and dissociation could be efficiently photoregulated. The difference in T(m) between trans- and cis-form (DeltaDeltaT(m)) was as large as 9 approximately 12 degrees C when azobenzene was tethered at the central position of a 10-nt-long RNA. Interestingly, photoregulation ability of the azobenzene introduced in RNA was even greater than that in DNA for photoswitching the corresponding duplex formation. The constructed photoresponsive RNA is promising to be applied for the photoregulation of RNA functions such as Ribozyme activity, RNAi, pre-mRNA editing, and aptamer complex formation.
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