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Updated: Jul 10, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Thermodynamics of dimerization of parallel quadruplex DNAs through the end-to-end stacking of 3'-terminal G-quartets
Noriko Sakuma1, Makie Ozawa, Shin Kawano
1Department of Chemistry, University of Tsukuba.Tsukuba 305-8571, Japan.
Abstract:
DNA sequence d(TTAGGG) spontaneously forms all-parallel G-quadruplex DNA in the presence of KV We have shown that G-quadruplex DNAs formed from the related sequences undergo dimerization through the intermolecular end-to-end stacking of the 3'-terminal G-quartets. 2' 3 We found that the coexistence of G-quadruplex DNAs formed from two different sequences results in the formation of "hetero-dimer", in addition to "homo-dimer". Thermodynamic characterization of the dimerization process revealed a compensatory relationship between the enthalpy and entropy changes of the process.
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