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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Photochemical properties of novel fluorescent probes for detection of DNA

Shuji Ikeda1, Akimitsu Okamoto

  • 1The Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), Wako, Saitama 351-0198, Japan.

Nucleic Acids Symposium Series (2004)
|November 22, 2007
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...