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Cooperative regulation of p73 promoter by Yin Yang 1 and E2F1

Shourong Wu1, Saomi Murai, Kazunori Kataoka

  • 1Department of Chemistry and Biotechnology, Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

Nucleic Acids Symposium Series (2004)
|November 22, 2007
PubMed

Insights

The transcriptional factor Yin Yang 1 (YY1) regulates p73 activity. Silencing YY1 decreases p73 transcription, while its overexpression enhances it, revealing YY1

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Cancer Biology

Background:

  • Yin Yang 1 (YY1) is a transcription factor with diverse biological roles.
  • The p53 family member, p73, is crucial in cellular processes like development and cancer.

Purpose of the Study:

  • To investigate the role of YY1 in regulating the transcriptional activity of p73.
  • To elucidate the mechanism of YY1-mediated regulation of p73.

Main Methods:

  • Gene silencing using siRNA expression vectors in U2OS cells.
  • Overexpression studies to assess YY1's effect on p73 activity.
  • Analysis of YY1 and E2F1 interaction in p73 promoter regulation.

Main Results:

  • YY1 silencing significantly decreased p73 transcriptional activity.
  • YY1 overexpression dose-dependently enhanced p73 transcriptional activity.
  • Synergistic cooperation and physical interaction between YY1 and E2F1 in regulating the p73 promoter were observed.

Conclusions:

  • YY1 is a key regulator of p73 transcriptional activity.
  • YY1 and E2F1 physically interact to modulate p73 gene expression.
  • These findings offer insights into YY1's role in development, differentiation, and cancer biology.

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