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Updated: Jul 10, 2026

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
Published on: August 1, 2016
Enzymatic incorporation of 2-amino-6-vinylpurine derivative
Shuhei Imoto1, Md Monsur Ali, Yingfu Li
1Institute of Multidisciplinary Research for Advanced Materials Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai-city, Miyagi, Japan.
Abstract:
Modified nucleotides are incorporated by enzymatic reactions into a DNA oligonulceotide to provide additional functionalities, which has shown potential applications in a wide variety of fields. A large number of modified nucleotide triphosphates have been incorporated to DNA or RNA by polymerases. We have previously described that the sulfide derivatives (1) of 2-amino-6-vinyl purine nucleoside (2, AVP) are activated within duplex to form cross-link toward cytidine selectively at the target site. In this paper, we describe the preparation of the triphosphate derivative of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl) purine nucleoside (5, dAVP(S Me)-TP), and its enzymatic incorporation to DNA.
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