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Updated: Jul 10, 2026

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
Published on: May 10, 2022
[Isolation of surface antigen of hepatites B virus]
Abstract:
The scheme of immunoaffinity isolation of surface antigen of hepatitis B virus is developed. Selection of monoclonal antibodies was carried out and conditions for synthesis of immunoaffinity matrix were optimized; specificity of binding of HBs-antigen with a sorbent is improved; conditions of washing of columns and elution of HBs-antigen are chosen. The yield of purified HBs-antigen obtained according to the developed scheme exceeded 90% and it allows to use such HBsAg for immunization of animals and application in immunoanalysis.
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