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Updated: Aug 10, 2026
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
[Monoclonal antibodies used to differentiate between poliovirus types 1 and 3]
V P Shirobokov1, L V Kopanitsa, I V Nikolaenko
1A.A. Bogomolets National Medical University, 13 Shevchenko Blvd., Kyiv, 01601, Ukraine.
Abstract:
A panel of mice hybrids was obtained, producing monoclonal antibodies (MCAB), which are capable to differentiate the vaccine and wild strains of polioviruses of the I and III types in the reaction of virus neutralization (RVN). The obtained MCAB activity was estimated on laboratory reference-strains and on the field isolates of polioviruses isolated in the territory of Ukraine in 1995-1998. The results of RVN with obtained MCAB were compared with the data of intratypical differentiation carried out with the use of standard MCAB from the Pasteur Institute as well as with results of the chain polymerization reaction (CPR) and immunoenzyme analysis (IEA) with cross-adsorbed sera. High correlation has been demonstrated between the results of the intratypical differentiation with obtained MCAB and data of the rest of the experiments.
Insights
New monoclonal antibodies (MCAB) effectively differentiate poliovirus vaccine and wild strains. This advancement aids in distinguishing virus types during neutralization reactions, crucial for polio surveillance.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Distinguishing between vaccine-derived and wild polioviruses is critical for polio eradication efforts.
- Accurate intratypical differentiation is essential for effective disease surveillance and response.
Purpose of the Study:
- To develop and characterize novel monoclonal antibodies (MCAB) capable of differentiating vaccine and wild strains of poliovirus types I and III.
- To evaluate the efficacy of these newly produced MCAB in virus neutralization reactions.
Main Methods:
- Production of monoclonal antibodies (MCAB) in hybrid mice.
- Assessment of MCAB activity using laboratory reference strains and field isolates of poliovirus (Ukraine, 1995-1998).
- Comparison of results with standard MCAB (Pasteur Institute), chain polymerization reaction (CPR), and immunoenzyme analysis (IEA).
Main Results:
- The developed MCAB demonstrated the ability to differentiate vaccine and wild strains of poliovirus types I and III in virus neutralization reactions (RVN).
- Activity of MCAB was confirmed on both reference strains and field isolates.
- High correlation was observed between the intratypical differentiation results obtained using the new MCAB and established methods (standard MCAB, CPR, IEA).
Conclusions:
- The novel monoclonal antibodies are effective tools for the intratypical differentiation of poliovirus strains.
- These MCAB can reliably distinguish between vaccine and wild poliovirus types I and III.
- The findings support the utility of these MCAB in polio surveillance and control programs.
