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Related Experiment Video

Updated: Jul 10, 2026

In Vitro SUMOylation Assay to Study SUMO E3 Ligase Activity
09:45

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Published on: January 29, 2018

Papillomaviruses and the host SUMOylation system.

Y-C Wu1, A F Deyrieux, V G Wilson

  • 1Department of Microbial and Molecular Pathogenesis, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843-1114, USA.

Biochemical Society Transactions
|November 23, 2007
PubMed
Summary

Papillomavirus E2 proteins undergo SUMOylation, a process that increases their levels. This viral protein also impacts host cell SUMOylation, revealing a complex interaction with the host system during epithelial differentiation.

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Published on: November 1, 2019

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Papillomaviruses are small DNA viruses linked to skin lesions and malignancy.
  • Their life cycle involves stratified epithelium differentiation, modulated by viral early proteins.
  • The viral E2 protein is crucial for papillomavirus replication and transcription.

Purpose of the Study:

  • To investigate the SUMOylation of papillomavirus E2 proteins.
  • To explore the relationship between E2 protein levels and SUMOylation.
  • To understand E2's influence on host cell SUMOylation machinery.

Main Methods:

  • Demonstration of E2 protein SUMOylation.
  • Analysis of intracellular E2 protein levels upon SUMOylation overexpression.
  • Investigation of E2's effect on the SUMOylation of C/EBP.

Main Results:

  • Papillomavirus E2 proteins are SUMOylated.
  • Overexpression of SUMOylation significantly increases intracellular E2 protein levels.
  • E2 protein influences the SUMOylation state of its binding partner, C/EBP.

Conclusions:

  • SUMOylation regulates E2 protein levels, potentially linked to keratinocyte differentiation.
  • E2 protein modulates the SUMOylation of host transcription factor C/EBP.
  • A complex interplay exists between papillomavirus E2 and the host SUMOylation system.