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Updated: Jul 9, 2026

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue
Published on: March 23, 2015
Cytochrome c biogenesis in mitochondria
P Giegé1, J M Grienenberger, G Bonnard
1Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du général Zimmer, 67084 Strasbourg, France.
Abstract:
As part of the respiratory chain, c-type cytochromes are essential electron transporters. They are characterized by the covalent attachment of a heme prosthetic group. The biogenesis of these proteins includes all the processes leading to this fixation. Yeast and animals have evolved a comparatively simple mechanism relying on cytochrome c heme lyases. In contrast, plant mitochondria have kept a maturation pathway inherited from their prokaryote ancestor. It involves Ccm proteins encoded in both the nuclear and the mitochondrial genomes of plants. These proteins compose a heme delivery pathway, include an ABC transporter, a redox protein and a putative heme lyase.
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