[Effect of diet on lipid peroxidation in selected tissues and blood serum of rats]

The purpose of research was to determine the effect of the diet with supplemented qualitatively and quantitatively different fats on lipid peroxidation in rats. The experiment was performed on Wistar male rats. The diets supplemented with 10% and 20% rape oil, butter, lard, and two kinds of margarine were administered. Control diet contained 5.4% of fat. The concentration of malonyldialdehyde (MDA) was determined in blood, brain, liver, kidney, lung, spleen and heart. It was found that diet supplemented with 10% of fat caused an increase of MDA level from 10 to 30% in the tissue and the diet with 20% of fat--40 to 100%. These values were dependent on the kind of tissue and fat. Diet supplemented with rape oil caused an increase of MDA level in heart 5 times as compared to control. In conclusion, the administration of the diet supplemented with fat intensify lipid peroxidation.