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Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Toxicity Testing in Animals01:23

Toxicity Testing in Animals

Toxicity tests in animals are grounded on two main assumptions: first, the effects observed in laboratory animals can be extrapolated to humans, especially when adjusted for body surface area; second, high-dose exposure in animals is essential to identify potential human hazards from lower doses. This is based on the quantal dose-response concept, which faces the challenge of extrapolating results from relatively few test animals to much larger human populations. For example, a 0.01% incidence...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

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Related Experiment Video

Updated: Jul 9, 2026

Use of Hematopoietic Stem Cell Transplantation to Assess the Origin of Myelodysplastic Syndrome
06:39

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Published on: October 3, 2018

Toxicity testing using hematopoietic stem cell assays.

E Clarke1, C Pereira, R Chaney

  • 1StemCell Technologies Inc, Vancouver, Canada.

Regenerative Medicine
|November 24, 2007
PubMed
Summary
This summary is machine-generated.

Hematopoietic colony-forming cell assays, used for over 40 years, are crucial for drug discovery. These in vitro methods offer clinically relevant data for evaluating compound toxicity, saving time and costs in development.

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Last Updated: Jul 9, 2026

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Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

Area of Science:

  • Hematology
  • Toxicology
  • Drug Discovery

Background:

  • In vitro assays have been instrumental in understanding hematopoiesis for decades.
  • These assays are experiencing a resurgence in academic and industrial drug discovery.
  • Primary cells from diverse hematopoietic tissues are used to generate high-content data.

Purpose of the Study:

  • To highlight the renewed importance of in vitro hematopoietic assays in drug discovery and development.
  • To emphasize the clinical relevance of hematopoietic colony-forming cell assays for toxicity evaluation.
  • To demonstrate the utility of these assays in improving decision-making processes and reducing costs.

Main Methods:

  • Utilizing primary cells from various hematopoietic tissues across multiple species.
  • Employing established in vitro hematopoietic colony-forming cell assays.
  • Generating high-content data for compound evaluation.

Main Results:

  • Hematopoietic colony-forming cell assays provide clinically relevant data for assessing compound toxicity.
  • These assays offer valuable information, even though in vitro conditions don't fully replicate the human body.
  • The assays can supplement or replace expensive in vivo studies.

Conclusions:

  • In vitro hematopoietic assays are vital tools for modern drug discovery and development.
  • These assays enhance the evaluation of potential drug toxicity, improving safety assessments.
  • Their application can lead to significant time and cost savings in the drug development pipeline.