Chemiluminescence flow-through biosensor for glucose with eggshell membrane as enzyme immobilization platform

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biosensor Technology

Background:

• Accurate glucose monitoring is crucial for diabetes management.
• Development of sensitive and stable biosensors is an ongoing research area.
• Chemiluminescence (CL) offers a sensitive detection method for biosensing applications.

Purpose of the Study:

• To develop a novel chemiluminescence (CL) flow-through biosensor for glucose detection.
• To immobilize glucose oxidase (GOD) and horseradish peroxidase (HRP) enzymes on eggshell membrane.
• To optimize immobilization conditions and evaluate biosensor performance.

Main Methods:

• Enzyme immobilization on eggshell membrane using glutaraldehyde cross-linker.
• Chemiluminescence detection involving luminol oxidation catalyzed by HRP.
• Optimization of immobilization parameters: time, enzyme ratio, and cross-linker concentration.
• Storage stability assessment at 4°C for 5 months.

Main Results:

• Successful development of a CL flow-through biosensor for glucose.
• Optimized immobilization conditions yielded a stable and sensitive sensor.
• The biosensor demonstrated good storage stability over 5 months.
• Achieved short response time, high sensitivity, and ease of operation.

Conclusions:

• The developed eggshell membrane-based CL biosensor is a promising tool for glucose determination.
• The biosensor exhibits excellent stability, sensitivity, and rapid response.
• Successful application in determining glucose levels in human serum validates its practical utility.