Differentiation of endothelial cells in avian embryos does not depend on gastrulation

Unincubated quail eggs were treated with Cytochalasin B. By this means, gastrulation of the blastodiscs was inhibited. Fragments of these blastodiscs were grafted into wings buds of chick embryos, and the differentiation fate of graft-derived cells was studied. Results show that only endothelial cells differentiate from the grafts. They were even found outside the graft site in vessels made up of a chimeric endothelium. It can be concluded that determination, differentiation and migration of endothelial cells does not depend on gastrulation.