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L-alanine transport by systems A and ASC in plasma membrane vesicles isolated from Ehrlich cells
P Luque1, J Márquez, I Núñez de Castro
1Laboratorio de Bioquímica, Facultad de Ciencias, Universidad de Málaga, Spain.
Abstract:
Plasma membrane vesicles were prepared from Ehrlich cells using two-phase system compartmentation. These vesicles accumulated L-alanine mainly by means of Na(+)-dependent transport systems A and ASC. The kinetic of both transport systems could be elucidated by specific inhibition of system A with methyl-aminoisobutyric acid.