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Updated: Jul 9, 2026

Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
Identification of DNA basepairing via tunnel-current decay
Jin He1, Lisha Lin, Peiming Zhang
1Biodesign Institute, Department of Chemistry and Biochemistry, Department of Physics, Arizona State University, Tempe, AZ 85287, USA.
Abstract:
We propose a new approach for reading the sequence of a DNA molecule passing between electrodes on a nanopore, using hydrogen bond-mediated tunneling signals. The base-electrode interaction is modeled using a nucleo-base-functionalized STM probe that is pulled away from a nucleoside monolayer. Watson-Crick recognition results in slow decay of the tunnel current, which is uniquely characteristic of the basepair in over half the reads. Thirteen independent reads would yield the desired 99.99% accuracy.
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