Related Experiment Video

Updated: Jul 9, 2026

DNA Vector-based RNA Interference to Study Gene Function in Cancer
13:10

DNA Vector-based RNA Interference to Study Gene Function in Cancer

Published on: June 4, 2012

Lentiviral vector-mediated RNAi and its use for cancer research

Hidetoshi Sumimoto1, Yutaka Kawakami

  • 1Keio University School of Medicine, Division of Cellular Signaling, Institute for Advanced Medical Research, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan. sumimoto@sc.itc.keio.ac.jp

Future Oncology (London, England)
|November 29, 2007
PubMed

Insights

RNA interference (RNAi) using HIV vectors delivers short hairpin RNAs (shRNAs) for gene silencing. These vectors are valuable tools for cancer research and potential gene therapy applications.

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Oncology

Background:

  • RNA interference (RNAi) is a powerful tool for gene function analysis and developing therapeutic strategies for diseases like cancer.
  • RNAi can be delivered via various methods, with Human Immunodeficiency Virus (HIV) vectors offering stable gene transduction in both replicating and quiescent cells.
  • HIV vectors have broad host tropism and are utilized in gene therapy research.

Purpose of the Study:

  • To describe the development of HIV vectors expressing short hairpin RNAs (shRNAs).
  • To highlight the application of these shRNA-expressing HIV vectors in cancer research.
  • To discuss the future perspectives of these vectors in cancer gene therapy.

Main Methods:

  • Development of HIV vectors engineered to express shRNAs.
  • Utilizing these vectors for post-transcriptional gene silencing of specific genes.
  • Application of the vectors to evaluate gene involvement in cancer development and malignancy.

Main Results:

  • HIV vectors efficiently deliver shRNAs for targeted gene silencing.
  • These vectors have been successfully used to investigate the role of various genes in cancer.
  • The developed vectors show promise for future cancer gene therapy.

Conclusions:

  • shRNA-expressing HIV vectors are effective tools for functional genomics in cancer research.
  • These vectors facilitate the evaluation of gene functions related to cancer development and progression.
  • HIV vector-mediated shRNA delivery holds potential for future cancer gene therapy strategies.

More Related Videos

Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice06:33

Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice

Published on: February 16, 2024

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...