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Updated: Jul 9, 2026
06:52
Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
Published on: November 1, 2019
Functional nucleic acids in high throughput screening and drug discovery
Seergazhi G Srivatsan1, Michael Famulok
1LIMES Institute, Program Unit Chemical Biology and Medicinal Chemistry, c/o Kekulé-Institut für Organische Chemie und Biochemie, Gerhard Domagk-Str. 1, D-53121 Bonn, Germany.
Combinatorial Chemistry & High Throughput Screening
|November 30, 2007
Summary
In vitro selection generates functional nucleic acids, like aptamers and ribozymes, for high-affinity molecular recognition. These signaling nucleic acids are crucial for developing sensitive detection reagents and screening formats.
Area of Science:
- Biochemistry
- Molecular Biology
- Biotechnology
Background:
- Functional nucleic acids, including aptamers and ribozymes, are key tools in molecular recognition.
- These molecules exhibit high affinity and specificity for various targets.
- Structural alterations upon recognition are often harnessed for signal generation.
Purpose of the Study:
- To summarize the development of different types of signaling nucleic acids.
- To review approaches for constructing signaling aptamers and ribozymes.
- To highlight their application as sensitive detection reagents.
Main Methods:
- In vitro selection techniques for generating functional nucleic acids.
- Rational design approaches for creating signaling aptamers and ribozymes.
- Development of screening formats for nucleic acid-based detection.
Main Results:
- Successful generation of signaling aptamers and ribozymes through design and selection.
- Demonstrated utility of these molecules as sensitive detection reagents.
- Established various approaches for implementing signaling nucleic acids in screening formats.
Conclusions:
- Signaling nucleic acids are versatile tools for molecular detection.
- In vitro selection and design are effective strategies for their development.
- These reagents offer sensitive and specific detection capabilities for diverse applications.
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