Crystallization and preliminary x-ray studies of the unliganded wild-type bovine thrombin
Daisuke Iyaguchi1, Min Yao, Nobuhisa Watanabe
1Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, 061-0293, Japan.
Abstract:
Wild type of bovine thrombin has been crystallized in a ligand-free form by the hanging drop vapor diffusion method with polyethylene glycol 4000 and 2-propanol. The crystals belong to space group P4 3 2 12 with unit cell parameters of a = b = 87.7 A, c = 195.9 A. X-ray diffraction data were collected to 2.8 A resolution.
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