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Purification and analysis of growth-regulating xyloglucan-derived oligosaccharides by high-pressure liquid
1Department of Botany, University of Edinburgh, Great Britain.
Abstract:
The retention times of 10 oligosaccharides, generated from the xyloglucans of Rosa and Tropaeolum by the action of Trichoderma cellulase, and of 17 related carbohydrates, in h.p.l.c. on an amino-substituted silica (Amino-Spheri-5) depended largely on the number of hydroxyl groups per molecule, whereas h.p.l.c. on a pellicular anion-exchange resin (CarboPac PA1) was strongly influenced by the nature of the sugar residues present, especially L-fucose, and by their linkages. The major nonasaccharide (XG9, D-Glc4-D-Xyl3-D-Gal-L-Fuc) obtained from Rosa xyloglucan, after purification on Amino-Spheri-5, retained biological activity as an inhibitor of auxin-induced growth in a Pisum stem-segment bioassay. H.p.l.c. on Amino-Spheri-5 was used to monitor the action of "Driselase" in stripping the non-reducing terminal alpha-D-Xylp-(1----6)-beta-D-Glcp units from XG9 to yield a pentasaccharide (XG5, D-Glc2-D-Xyl-D-Gal-L-Fuc).