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Evaluation for protein binding affinity of maghemite and magnetite nanoparticles
Se Chan Kang1, Yong Jun Jo, Jong Phil Bak
1Gyeonggi Pharmaceutical Research Center, Gyeonggi Bio-Center, Suwon 443-766, Korea.
Abstract:
We investigated the protein binding affinity of magnetite (Fe3O4) and maghemite (gamma-Fe2O3) nanoparticles with against non-characterized protein from human lung cancer A549 cell line on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The binding ability of maghemite was 400 ng/mg. According to the SDS-PAGE results, the protein binding affinity of maghemite nanoparticles is stronger than magnetite nanoparticles. These data suggest that a protein can be detected with maghemite nanoparticles.
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