Related Experiment Video
Updated: Jul 9, 2026

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
Published on: June 25, 2015
Meganuclease and transposon mediated transgenesis in medaka
Clemens Grabher1, Joachim Wittbrodt
1Pediatric Oncology, Dana-Farber Cancer Institute, Binney St, Boston, Massachusetts 02115, USA. Clemens_grabher@dfci.harvard.edu
Abstract:
From among a plethora of various gene delivery methods, the researcher must choose the right one according to availability for a given species and the precise application the transgenic animal is intended for. Here we review the progress in meganuclease and Sleeping Beauty transposon mediated transgenesis over recent years with a focus on medaka and zebrafish. We present a side-by-side comparison of these two approaches based on their biologic properties and provide interesting perspectives for future experiments and applications, which are different for the two techniques because of their distinct modes of action.
Related Concept Videos
Transposons
DNA-only Transposons
The donor site from where the transposon is excised is either degraded or...
In-vitro Mutagenesis
Overview of Transposition and Recombination
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...

