Related Experiment Video
Updated: Jul 9, 2026
09:38
Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
Published on: October 16, 2016
Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis
Yasunori Sasakura1, Yuichi Oogai, Terumi Matsuoka
1Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, 415-0025, Japan. sasakura@kurofune.shimoda.tsukuba.ac.jp
Genome Biology
|December 6, 2007
Abstract:
Achievement of transposon mediated germline transgenesis in a basal chordate, Ciona intestinalis, is discussed. A Tc1/mariner superfamily transposon, Minos, has excision and transposition activities in Ciona. Minos enables the creation of stable transgenic lines, enhancer detection, and insertional mutagenesis.
Related Concept Videos
02:57DNA-only Transposons
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
00:53Transgenic Organisms
Overview