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Tol2: a versatile gene transfer vector in vertebrates.

Koichi Kawakami1

  • 1Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, The Graduate University of Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan. kokawaka@lab.nig.ac.jp

Genome Biology
|December 6, 2007
PubMed
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The Tol2 element from medaka fish is a versatile transposon system. It facilitates efficient gene insertion in various vertebrate cells, enabling advanced transgenic studies.

Area of Science:

  • Genetics
  • Molecular Biology
  • Transposon Technology

Background:

  • The Tol2 element is an autonomous transposon originating from medaka fish.
  • It encodes a functional transposase enzyme essential for its activity.
  • The system is characterized by specific terminal and subterminal DNA sequences crucial for transposition.

Purpose of the Study:

  • To review the applications of the Tol2 transposon system in vertebrate transgenic studies.
  • To discuss the mechanism and capabilities of the Tol2 transposase.
  • To explore potential future applications of Tol2 in research.

Main Methods:

  • Utilizing the Tol2 transposase to mediate the integration of DNA constructs.
  • Cloning large DNA inserts (up to 11 kilobases) within the Tol2 construct.

Related Experiment Videos

  • Testing the activity of the Tol2 system across various vertebrate cell types.

    • Main Results:

    • The Tol2 transposase efficiently catalyzes transposition of constructs with defined left and right end sequences.
    • Large DNA inserts do not significantly impede Tol2 transpositional activity.
    • The Tol2 system demonstrates broad activity in diverse vertebrate cells, including zebrafish, Xenopus, chicken, mouse, and human.

    Conclusions:

    • The Tol2 transposon system is a powerful and adaptable tool for transgenic research in vertebrates.
    • Its ability to accommodate large inserts and its broad cell activity make it highly valuable.
    • Future applications of Tol2 are promising for advancing genetic studies in various species.