Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Allergic Reactions

Allergic Reactions

Allergic Drug Reactions

Allergic Drug Reactions

Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing numerous...

Allergic Reactions: Anaphylaxis

Allergic Reactions: Anaphylaxis

Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin, heparin),...

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Cross-reactivity

Cross-reactivity

Asthma: Pathogenesis and Management

Asthma: Pathogenesis and Management

Asthma is a chronic pulmonary condition involving inflammation of the airways, hyper-reactivity, and reversible obstruction of the airways. This condition can significantly impact a person's quality of life, making breathing difficult and leading to distressing symptoms.
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Allergic Rhinitis and its Impact on Asthma (ARIA) Classes in MASK-air Users.

Journal of investigational allergology & clinical immunology·2025

Same author

Triple inhaled therapy in asthma: Beliefs, behaviours and doubts.

Pulmonary pharmacology & therapeutics·2024

Same author

Adherence to inhaled corticosteroids and long-acting β2-agonists in asthma: A MASK-air study.

Pulmonology·2023

Same author

Rhinitis associated with asthma is distinct from rhinitis alone: The ARIA-MeDALL hypothesis.

Allergy·2023

Same author

[Prevalence and incidence of ictus in Europe: systematic review and meta-analysis].

Anales del sistema sanitario de Navarra·2021

Same author

Exploratory dietary patterns and cognitive function in the "Seguimiento Universidad de Navarra" (SUN) Prospective Cohort.

European journal of clinical nutrition·2021

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Efficient bibliographic searches on allergology using PubMed.

J M Sáez Gómez¹, E Aguinaga Ontoso, J M Negro Alvarez

¹Department of Social and Health Sciences. University of Murcia Medical School. Spain. jmsaez@um.es

Allergologia Et Immunopathologia

|December 1, 2007

Summary

Learn how to effectively search PubMed, a vital biomedical database, for allergology research. This guide covers search strategies for efficient and precise results in biomedical literature searches.

Related Experiment Videos

Last Updated: Jul 9, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E (sIgE)

Published on: April 21, 2019

Area of Science:

Biomedical Literature and Databases
Information Science

Background:

PubMed is a primary, non-specialized database for biomedical literature, updated internationally and maintained by the U.S. government.
It integrates multiple subgroups, including MEDLINE, allowing simultaneous searching.
While comprehensive, PubMed's content is predominantly English-language, necessitating supplementary sources for specific regions or specialties.

Purpose of the Study:

To outline PubMed's key features and optimal search techniques for allergology research.
To enhance the efficiency and accuracy of information retrieval within the PubMed database.

Main Methods:

Utilizing natural language searching with Automatic Term Mapping for broader sensitivity.
Employing the Medical Subject Headings (MeSH) thesaurus for precise descriptor translation and high specificity.
Leveraging Boolean operators (OR, AND, NOT), field tags, Limits, and Clinical Queries for refined search strategies.

Main Results:

Automatic Term Mapping enhances search sensitivity by linking natural language to descriptors, though specificity may be reduced.
MeSH thesaurus integration and advanced search operators (AND, OR, NOT) significantly improve search specificity.
Clinical Queries facilitate direct clinical application by identifying systematic reviews, meta-analyses, and clinically focused papers.

Conclusions:

Effective PubMed searching requires a combination of natural language and structured approaches (MeSH) for optimal results.
Advanced features like Clinical Queries, search history, and article clustering tools (Related Articles) further refine information discovery.
Strategic use of PubMed's functionalities ensures efficient retrieval of relevant biomedical literature, particularly in specialized fields like allergology.