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Updated: Jul 9, 2026

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
Published on: December 16, 2019
Technique for detecting changes in fluorescence lifetime by means of optoelectronic circuit auto-oscillation
Abstract:
We present a new, simple, inexpensive, and highly precise approach to excited-state fluorescence-lifetime-based measurements. The detection system consists of a closed-loop optoelectronic arrangement containing a radio frequency resonance amplifier, a fluorescence excitation light source, a fiber-optic delay line, and a photodetector. The system exhibits auto-oscillations in the form of intensity modulation. The oscillation frequency varies with the modulation phase shift of the fluorescent light. This frequency is used as the detection parameter, which is advantageous because frequency may be measured easily, inexpensively, and with high precision. This technique is well suited for chemical or biosensor applications.
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