[Fluorescence microscopic study of environmental water sample by a self-ordered ring technique with microwave...

Area of Science:

• Analytical Chemistry
• Environmental Science

Background:

• Picric acid is a hazardous pollutant requiring sensitive detection methods.
• Traditional methods for picric acid analysis in water often lack sensitivity and are prone to interference.

Purpose of the Study:

• To develop a highly sensitive and reliable method for detecting picric acid in environmental water samples.
• To utilize a self-ordered ring (SOR) technique combined with microwave heating for enhanced analysis.

Main Methods:

• A self-ordered ring (SOR) was formed on a glass slide using microwave heating in a specific reaction medium.
• Berberine fluorescence was quenched by picric acid, with intensity measured to quantify picric acid content.
• The method was applied to natural and synthetic water samples.

Main Results:

• The maximum fluorescence intensity (Imax) showed a linear relationship with picric acid concentration.
• Detection limits as low as 1.3 x 10(-8) mol/L were achieved for picric acid.
• Recoveries ranged from 96.3% to 108.0% with relative standard deviation (RSD) below 3.3% in water samples.
• Sensitivity was enhanced 50-60 times compared to traditional spot or solution analyses, with reduced background interference.

Conclusions:

• The proposed microwave-assisted SOR technique offers a highly sensitive, rapid, and reliable method for picric acid determination in environmental water.
• This technique demonstrates significant advantages over existing methods, including enhanced sensitivity and reduced interference.
• The SOR technique shows great potential for practical applications in environmental monitoring and biochemical analysis.