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Split Retina as an Improved Flatmount Preparation for Studying Inner Nuclear Layer Neurons in Vertebrate Retina
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Retinal nerve fiber layer split bundles are true anatomic variants.

Ehud Kaliner1, Matan J Cohen, Hagai Miron

  • 1Department of Ophthalmology, Hadassah-Hebrew University Medical Center, Jerusalem, Israel.

Ophthalmology
|December 7, 2007
PubMed
Summary

Retinal nerve fiber layer (RNFL) split bundles, previously seen in scans, are confirmed as a real anatomical feature in normal human eyes through histologic analysis. This finding helps differentiate normal variants from actual RNFL loss.

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Area of Science:

  • Ophthalmology
  • Anatomy
  • Histology

Background:

  • Retinal nerve fiber layer (RNFL) split bundles have been observed in normal eyes using scanning laser polarimetry.
  • The nature of these split bundles as either an imaging artifact or a true anatomical finding required further investigation.

Purpose of the Study:

  • To histologically ascertain the presence of RNFL split bundles in normal human eyes.
  • To determine if RNFL split bundles represent a genuine anatomical variation.

Main Methods:

  • Histologic examination of 14 normal postmortem human eyes.
  • Measurement of peripapillary RNFL thickness at specific diameters (3.0, 3.5, 4.0, 4.5 mm).
  • Assessment for superior or inferior RNFL split bundle patterns.

Main Results:

  • Histologic evidence of RNFL split bundles was found in 5 out of 14 eyes (35.7%).
  • Split bundles were observed in the superior quadrant in 3 eyes and the inferior quadrant in 2 eyes.
  • No eyes exhibited both superior and inferior definite split bundles simultaneously.

Conclusions:

  • This study provides histologic evidence that RNFL split bundles are a true anatomical finding, not an artifact of imaging techniques.
  • The presence of RNFL split bundles appears to be a relatively common normal variant in human eyes.
  • It is crucial to distinguish the presence of a split bundle, as a normal variant, from actual RNFL loss in clinical assessments.