Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Centrioles and Centrosomes01:13

Centrioles and Centrosomes

Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Cell Diversity01:13

Cell Diversity

The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular organisms...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A closely related pair of superoxide dismutase isozymes from Staphylococcus aureus show distinct stabilities and proton-exchange dynamics.

The Journal of biological chemistry·2026
Same author

A developmental condensin I complex assists the Paramecium PiggyMac domesticated transposase during programmed DNA elimination.

Nucleic acids research·2026
Same author

The tiny germline chromosomes of Paramecium aurelia have an exceptionally high recombination rate and are capped by a new class of Helitrons.

BMC biology·2026
Same author

HaDeX2: multi-dimensional analysis of hydrogen-deuterium exchange mass spectrometry data.

Bioinformatics (Oxford, England)·2026
Same author

The in vitro effect of omentin-1 on the global proteome of granulosa cells from normal weight Large White and fat Meishan pigs.

Journal of proteomics·2026
Same author

Solution 3D structure and conformational flexibility of the endothelial monocyte activating polypeptide II (EMAP II) revealed by NMR spectroscopy and molecular dynamics simulations.

Journal of structural biology·2025

Related Experiment Video

Updated: Jul 9, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Functional diversification of centrins and cell morphological complexity.

Delphine Gogendeau1, Catherine Klotz, Olivier Arnaiz

  • 1CNRS, Centre de Génétique Moléculaire, UPR 2167, Gif-sur-Yvette, F-91198, France. gogendeau@cgm.cnrs-gif.fr

Journal of Cell Science
|December 7, 2007
PubMed
Summary

Centrins and Sfi1p-like proteins are essential for the assembly and function of the infraciliary lattice (ICL) in Paramecium. This study reveals their diverse roles in ICL architecture and organelle positioning.

More Related Videos

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
15:41

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 2, 2010

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Related Experiment Videos

Last Updated: Jul 9, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
15:41

Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells

Published on: December 2, 2010

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Area of Science:

  • Cell Biology
  • Proteomics
  • Biochemistry

Background:

  • Centrins are crucial for microtubule organizing center (MTOC) duplication and form contractile arrays.
  • Sfi1p, a centrin partner, has multiple binding sites, suggesting a model for centrin-mediated contractility and MTOC duplication.
  • Paramecium's infraciliary lattice (ICL) is an extended contractile array composed of centrins and a Sfi1p-like protein, PtCenBP1p.

Purpose of the Study:

  • To investigate the functional redundancy and roles of diverse centrin and Sfi1p-like proteins in the Paramecium ICL.
  • To understand the molecular and supramolecular architecture of the ICL.
  • To explore the function of conserved centrins in coordinating organelle duplication and positioning.

Main Methods:

  • Proteomic analysis of the Paramecium ICL.
  • In vivo functional studies of centrin and Sfi1p-like protein subfamilies.

Main Results:

  • The ICL proteome includes ten centrin subfamilies and two Sfi1p-like protein subfamilies.
  • All identified centrin and Sfi1p-like protein subfamilies are essential for ICL biogenesis.
  • ICL-specific proteins contribute to the ICL's molecular and supramolecular architecture.
  • A conserved centrin subfamily is found at the ICL, basal bodies, and contractile vacuole pores, potentially coordinating organelle duplication.

Conclusions:

  • The diversity of centrin and Sfi1p-like proteins reflects specialized roles in ICL assembly and function.
  • Specific centrin and Sfi1p-like proteins are vital for the structural integrity of the ICL.
  • A conserved centrin subfamily may play a broader role in the regulation of cortical organelle dynamics.