Related Experiment Video
Updated: Mar 18, 2026

A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
HaDeX2: multi-dimensional analysis of hydrogen-deuterium exchange mass spectrometry data
Weronika Puchała1, Krystyna Grzesiak2,3, Dominik Rafacz4
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-106 Warsaw, Poland.
Summary:
Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) monitors deuterium uptake at the peptide level, in a time-dependent manner. It produces complex, multi-dimensional data that must be interpreted at minimum both the temporal and sequence levels. Specialized tools are therefore essential to preprocess, integrate, and analyze HDX-MS data and translate it into meaningful biological insights. HaDeX2 provides statistical inferences and their visualizations across five dimensions of HDX-MS data: protein sequence, time, biological states, peptide charge and experimental replicates.
Availability And Implementation:
HaDeX2 is freely available as an R package (https://github.com/hadexversum/HaDeX2; https://doi.org/10.5281/zenodo.18543703) and web server (https://hadex2.mslab-ibb.pl/). To run the GUI locally, users should install a dedicated companion package (https://github.com/hadexversum/HaDeXGUI).
Related Concept Videos
¹H NMR of Labile Protons: Deuterium (²H) Substitution
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
2D NMR: Overview of Heteronuclear Correlation Techniques
High-Resolution Mass Spectrometry (HRMS)

