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Updated: Jul 9, 2026

Optimizing Isolation and Purification of Murine Glomerular Mesangial Cells
Published on: March 7, 2025
Chemically modified heparin inhibits mesangial cell proliferation induced by high glucose through interfering with
Deng-Ke Yin1, Wen-Bing Yao, Xiang-Dong Gao
1School of Life Science and Technology, China Pharmaceutical University, 24 Tongjia Road, Nanjing City 210009, China.
Abstract:
The aims of this study were to investigate whether chemically modified non-anticoagulation heparin derivate (Periodate-Oxidized/Borohydride-Reduced modified heparin (OR-heparin)) can inhibit high glucose-induced human mesangial cell proliferation and its influence on the cell cycle. OR-heparin with low anticoagulation activity inhibited high glucose-induced early proliferation in a dose-dependent manner. OR-heparin released high glucose-arrested mesangial cells at G(1) phase, and dose-dependently increased S phase. OR-heparin also inhibited high glucose-activated ERK1/2 phosphorylation, induced p27(Kip1) expression, and suppressed reactive oxygen species (ROS) accumulation in a dose-dependent manner. Our results suggest that OR-heparin releases high glucose-arrested cells on G(1) phase and inhibits high glucose-induced mesangial cell proliferation through blocking ERK1/2 phosphorylation and delaying S phase progression, which may be in correlation with OR-heparin suppressing ROS accumulation.
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