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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

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Related Experiment Video

Updated: Jul 9, 2026

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
09:57

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

[Development of high efficiency separation method for biomolecules].

Masaru Kato1

  • 1Center for NanoBio Integration, Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Room 251a, The Faculty of Engineering Building No 3, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. kato@cnbi.t.u-tokyo.ac.jp

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|December 7, 2007
PubMed
Summary

Separating mixed samples like bio- and environmental samples is crucial. This study presents advanced separation techniques for analyzing target compounds and evaluating nanomaterials efficiently.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Context:

  • Accurate analysis of target compounds in complex bio- and environmental samples is essential.
  • Developing efficient separation and purification methods for novel nanomaterials is a significant challenge.
  • Existing analytical techniques often require large sample volumes and extensive analysis times.

Purpose:

  • To introduce novel, highly efficient, and selective separation methods for complex samples.
  • To address the need for effective separation and evaluation techniques for nanomaterials.
  • To showcase recent advancements in integrated separation technologies and biomolecule applications.

Summary:

  • Developed high-resolution, selective columns and efficient analytical methods using integrated techniques and biomolecules.
  • These methods reduce analytical time and sample volume requirements.
  • The review focuses on recent achievements in the separation of nano-scale materials.

Impact:

  • Enables faster and more accurate analysis of target compounds in diverse sample matrices.
  • Provides crucial tools for the purification, evaluation, and application of advanced nanomaterials.
  • Advances the field of analytical chemistry with innovative separation technologies.