Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probing cellular microenvironments and tissue remodeling by atomic force microscopy.

Thomas Ludwig1, Robert Kirmse, Kate Poole

  • 1Group Microenvironment of Tumor Cell Invasion, German Cancer Research Center, BIOQUANT-Zentrum; BQ 0009 NWG Ludwig, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany. t.ludwig@dkfz.de

Pflugers Archiv : European Journal of Physiology
|December 7, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Volume and surface methods for microparticle traction force microscopy: a computational and experimental comparison.

Soft matter·2026
Same author

Dynamic switching of cell-substrate contact sites allows gliding diatoms to modulate the curvature of their paths.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Compressive stress-driven Piezo1 activation and Rho-ROCK mechanotransduction promote tumor progression via epigenetic mechanical memory.

Science advances·2026
Same author

Rosette margination in blood flow during malaria pathogenesis.

Biophysical journal·2025
Same author

Lenacapavir-induced capsid damage uncovers HIV-1 genomes emanating from nuclear speckles.

The EMBO journal·2025
Same author

Modeling mechanochemical coupling in optogenetically activated cell layers.

Biophysical journal·2025
Same journal

Expression of protein kinase A catalytic subunits in healthy and diseased mouse kidneys.

Pflugers Archiv : European journal of physiology·2026
Same journal

Localization of O₂‑sensing ADO‑RGS pathway components in mouse and human kidneys under normoxia, hypoxia and renal fibrosis.

Pflugers Archiv : European journal of physiology·2026
Same journal

Cholesterol-depleting dextrin depolarizes sarcolemma: the contribution of sodium / calcium channels and membrane integrity.

Pflugers Archiv : European journal of physiology·2026
Same journal

Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates fibrosis progression.

Pflugers Archiv : European journal of physiology·2026
Same journal

Renal NHE3 is required to limit hypokalemia and metabolic acidosis during dietary potassium deficiency.

Pflugers Archiv : European journal of physiology·2026
Same journal

Xenin-25 improves indomethacin-induced acute gastric injury in rats.

Pflugers Archiv : European journal of physiology·2026
See all related articles

Cellular functions depend on the extracellular microenvironment. Atomic Force Microscopy (AFM) reveals how cells sense and remodel their surroundings, crucial for tissue repair and cancer invasion.

Area of Science:

  • Cell Biology
  • Biophysics
  • Biotechnology

Background:

  • Cellular functions are significantly influenced by the extracellular microenvironment's biophysical properties, such as stiffness and fiber orientation.
  • Cell adhesion and migration are critical for tissue maintenance, wound repair, cancer invasion, and morphogenesis.
  • Cells actively sense and remodel their surroundings using strategies like pericellular proteolysis and adaptive migration tactics.

Purpose of the Study:

  • To review common principles of tissue remodeling.
  • To highlight the application of Atomic Force Microscopy (AFM) techniques in studying cell-environment interactions.
  • To emphasize the need for combined AFM force spectroscopy for a comprehensive understanding.

Main Methods:

  • Atomic Force Microscopy (AFM) for nanoscale probing and high-resolution imaging of live cells.

Related Experiment Videos

  • AFM techniques including elasticity mapping, combined AFM and fluorescence microscopy, and morphometric imaging of proteolytic activity.
  • Force spectroscopy applications on single molecules and individual cells.

    • Main Results:

    • AFM provides access to critical biophysical and biochemical determinants of cell-environment interactions.
    • Various AFM techniques allow for detailed analysis of cellular remodeling strategies.
    • Combined AFM force spectroscopy is essential for a complete understanding of these processes.

    Conclusions:

    • Understanding cell-environment interactions requires detailed biophysical and biochemical analysis.
    • AFM is a powerful tool for investigating cellular sensing and remodeling mechanisms.
    • Integrating AFM force spectroscopy with other techniques is crucial for advancing knowledge in this field.