Related Experiment Video
Updated: Jul 9, 2026
09:35
Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle
Published on: February 1, 2017
[Expression and preliminary applications of hepatitis B surface antigen mutants]
Chang-Cheng Zhu1, Chun-Zhen Luo, Man Wang
1Biochemistry and Molecular Biology, East China University of Science and Technology, Shanghai 200237, China. changchengzh@163.com
Summary
This study successfully cloned and expressed a Hepatitis B surface antigen (HBsAg) mutant in Pichia pastoris. The recombinant HBsAg mutant demonstrated immunoreactivity, showing practical value for diagnostic assay quality control.
Area of Science:
- Molecular biology and recombinant protein expression.
- Biotechnology and microbial expression systems.
Context:
- Hepatitis B virus (HBV) surface antigen (HBsAg) is crucial for diagnostic assays.
- Developing efficient expression systems for HBsAg mutants is important for vaccine development and diagnostics.
Purpose:
- To clone and express a mutant form of HBsAg in the Pichia pastoris yeast expression system.
- To generate and characterize recombinant HBsAg mutants for potential diagnostic applications.
Summary:
- A modified pGAP-MS expression vector was constructed and integrated into Pichia pastoris GS115.
- Stable, high-copy number integrants were selected and expressed the HBsAg mutant protein.
- Expression was confirmed via SDS-PAGE and Western blot, revealing a 38 kDa protein, and all 10 mutants were reactive in diagnostic assays.
Impact:
- Successful expression of immunoreactive HBsAg mutants in Pichia pastoris.
- The recombinant HBsAg mutant has practical utility in the quality control and clinical application of commercial diagnostic assays.
Related Concept Videos
01:25Hepatitis
Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
01:28Viral Hepatitis I: Introduction
Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...