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Related Experiment Video

Updated: Jul 9, 2026

Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature

Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature

Published on: January 24, 2025

[Conjunctival stamp in diabetic patients].

Rodica Mnescu¹, Mihaela Niculescu, Doina Barascu

¹Catedra de anatomie, UMF Craiova.

Oftalmologia (Bucharest, Romania : 1990)

|December 11, 2007

Summary

Diabetic patients often experience dry eye syndrome due to reduced tear production and changes in conjunctiva cells. Advanced diabetes and age exacerbate these effects, impacting ocular health.

Area of Science:

Ophthalmology
Diabetology
Cell Biology

Background:

Diabetes mellitus is associated with various ocular complications.
Dry eye syndrome (lacrimal hyposecretion) is a common complaint among diabetic patients.
Conjunctival changes may serve as an indicator of ocular surface disease in diabetes.

Purpose of the Study:

To investigate conjunctival stamp alterations in diabetic patients.
To correlate these changes with ocular surface parameters and diabetes duration.
To assess the prevalence of dry eye syndrome in the studied diabetic cohort.

Main Methods:

Ophthalmological examinations including Schirmer and Tear Break-Up Time (BUT) tests were performed on diabetic patients (50-80 years old) and controls.
Conjunctival stamps were collected, fixed, Giemsa stained, and analyzed via photonic microscopy.

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Histopathological evaluation of epithelial and calyciforme cells in conjunctival stamps was conducted.

Main Results:

Schirmer and BUT tests indicated lacrimal hyposecretion in a majority of diabetic patients.
Conjunctival stamps from diabetic patients showed significant alterations in epithelial and calyciforme cells.
Older diabetic patients with longer disease duration exhibited more pronounced conjunctival stamp changes.

Conclusions:

Diabetic patients frequently present with dry eye syndrome, evidenced by lacrimal hyposecretion.
Conjunctival stamp analysis reveals cellular changes indicative of ocular surface damage in diabetes.
Conjunctival stamp examination can aid in diagnosing dry eye syndrome, particularly in elderly patients with long-standing diabetes.