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Related Concept Videos

Stress Concentrations01:13

Stress Concentrations

The concept of stress concentration is crucial for understanding how materials respond under bending stresses, particularly when there are irregularities or discontinuities in the material's geometry. Normally, stress in a symmetric member subjected to pure bending is assumed to be uniformly distributed across the entire cross-section. However, this assumption does not hold when there are variations in the cross-sectional geometry or the presence of notches and holes.
The stress concentration...
Stress Concentrations01:24

Stress Concentrations

Stress concentration is when stress intensifies near discontinuities such as holes or abrupt cross-sectional changes in a structural member. This localized stress can often surpass the average stress within the member. The stress distribution in flat bars, either with a circular hole or varying widths connected by fillets, can be determined experimentally using a photoelastic method. The results are based on ratios of geometric parameters like the ratio of the hole's radius to the smaller width...
Stress: General Loading Conditions01:15

Stress: General Loading Conditions

To grasp the intricacy of real-world conditions where multiple loads are applied simultaneously to a structure, one might visualize a section passing through a specific point within a body, aligned parallel to the xy plane. This section is subjected to various forces, including original loads, normal forces, and shearing forces.
The shearing force, possessing potential directionality within the plane of the section, is simplified into two component forces running parallel to the x and y axes.
Stress Concentrations in Circular Shafts01:18

Stress Concentrations in Circular Shafts

Consider the elastic torsion formula, which applies to a circular shaft with a consistent cross-section. This formula assumes that the shaft's ends are loaded with rigid plates firmly attached. However, in many cases, torques are applied to the shaft through mechanisms like flange couplings or gears, which are connected by keys inserted into keyways. This application method modifies the stress distribution near the point of torque application, causing it to deviate from the distributions...
Stress01:20

Stress

When a force is applied on a body, it undergoes deformation. In order to restore the body to its original shape and/or size, an opposite or restoring force is generated within the body. This restoring force is equal to the magnitude of the applied force, but acts in the opposite direction. The amount of this restoring force developed per unit area of the body is called stress. Stress is a tensor quantity and has the SI unit pascal. Stress can be separated into four broad categories depending...
Components of Stress01:23

Components of Stress

Stress analysis under multiple loading conditions is intricate, necessitating a comprehensive grasp of normal and shearing stresses. Consider a small cube at point O, subjected to stress on all six faces, visible or not. Normal stress components σx, σy, σz act perpendicularly to the x, y, and z axes. Shearing stress components τxy and τxz are exerted on faces perpendicular to these axes.
Interestingly, the hidden cube faces also experience these stresses, equal and opposite to those on the...

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Related Experiment Video

Updated: Jul 9, 2026

A Cost-effective and Reliable Method to Predict Mechanical Stress in Single-use and Standard Pumps
07:34

A Cost-effective and Reliable Method to Predict Mechanical Stress in Single-use and Standard Pumps

Published on: August 5, 2015

Gravitation-driven stress-reduced cell handling.

Michael Boettcher1, Magnus Jaeger, Michael Kirschbaum

  • 1Fraunhofer Institute for Biomedical Engineering IBMT, Am Muehlenberg 13, 14476, Potsdam, Germany.

Analytical and Bioanalytical Chemistry
|December 11, 2007
PubMed
Summary

We developed a simple lab-on-chip device for cell handling using sedimentation and dielectrophoresis. This technology enables precise cell sorting and trapping with low stress, offering an economical solution for cellular biotechnology.

More Related Videos

Handling Techniques to Reduce Stress in Mice
09:03

Handling Techniques to Reduce Stress in Mice

Published on: September 25, 2021

Related Experiment Videos

Last Updated: Jul 9, 2026

A Cost-effective and Reliable Method to Predict Mechanical Stress in Single-use and Standard Pumps
07:34

A Cost-effective and Reliable Method to Predict Mechanical Stress in Single-use and Standard Pumps

Published on: August 5, 2015

Handling Techniques to Reduce Stress in Mice
09:03

Handling Techniques to Reduce Stress in Mice

Published on: September 25, 2021

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cellular Engineering

Background:

  • Handling delicate cell samples, such as stem cells, requires precise and gentle methods.
  • Existing dielectrophoresis systems can induce high stress on cells due to external pumping mechanisms.
  • There is a need for cost-effective and efficient microfluidic solutions for cellular analysis.

Purpose of the Study:

  • To present a novel lab-on-chip device for the manipulation of small, delicate cell samples.
  • To demonstrate the device's capability for cell trapping and sorting using a combination of sedimentation and dielectrophoresis.
  • To highlight the low-stress environment provided by the device for cell processing.

Main Methods:

  • Utilizing a lab-on-chip platform integrating sedimentation (gravity-driven transport) and dielectrophoresis (radio-frequency electric fields).
  • Employing electrodes within microchannels to generate particle-selective forces based on size and polarizability.
  • Designing the device with integrated injection and sampling ports, eliminating the need for external pumps.

Main Results:

  • Demonstrated contact-free cell trapping and selective cell/particle sorting capabilities.
  • Successfully separated pairs of human cells and antibody-coated beads (relevant to T cell activation) from unbound beads.
  • Achieved low cell stress levels during manipulation, outperforming traditional dielectrophoresis systems reliant on external pumping.

Conclusions:

  • The developed lab-on-chip device offers a versatile and simple solution for handling delicate cell samples.
  • The technology enables efficient cell sorting and trapping with minimal stress, suitable for applications in cellular biotechnology.
  • The device presents an economically viable alternative for microfluidic cell processing.