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Related Experiment Video

Updated: Jul 9, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

Published on: October 15, 2018

5-methylcytosine-selective osmium oxidation.

Akimitsu Okamoto¹

¹Frontier Research System, RIKEN, Wako, Saitama, Japan. aki-okamoto@riken.jp

Nucleosides, Nucleotides & Nucleic Acids

|December 11, 2007

Summary

Osmium oxidation efficiently modifies methylcytosine. This method enables site-specific detection of cytosine methylation status in DNA sequences using guide DNA hybridization.

Area of Science:

Biochemistry
Molecular Biology
Genetics

Background:

DNA methylation is a crucial epigenetic modification.
Accurate detection of DNA methylation is vital for understanding gene regulation.
Current methods for site-specific methylation detection can be complex.

Purpose of the Study:

To develop an efficient method for methylcytosine modification.
To enable site-specific detection of cytosine methylation status.
To utilize osmium oxidation for DNA methylation analysis.

Main Methods:

Osmium oxidation of methylcytosine.
Hybridization with a guide DNA sequence.
Formation of a bulge structure for detection.

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Last Updated: Jul 9, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase

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Published on: October 15, 2018

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Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

Published on: September 7, 2017

Main Results:

Methylcytosine was efficiently and easily modified via osmium oxidation.
The method allows for selective oxidation of methylcytosine.
Successful application in detecting cytosine methylation status at specific sites.

Conclusions:

Osmium oxidation provides a straightforward approach for methylcytosine modification.
This technique is applicable for site-specific DNA methylation detection in long sequences.
The formation of a bulge structure aids in the detection process.