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Updated: Jul 9, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Novel method for the synthesis of 2' -phosphorylated oligonucleotides
Darya S Novopashina1, Olesya S Totskaya, Maria I Meschaninova
1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
Abstract:
We have developed a new method for the preparation of oligodeoxyribonucleotides and oligo(2'-O-methylribonucleotides) that contain a 2'-phosphorylated ribonucleoside residue, and optimized it to avoid 2' -3' -isomerization and chain cleavage. Structures of the 2' -phosphorylated oligonucleotides were confirmed by MALDI-TOF MS and enzymatic digestion, and the stability of their duplexes with DNA and RNA was investigated. 2'-Phosphorylated oligonucleotides may be useful intermediates for the introduction of various chemical groups for a wide range of applications.
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