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The macroPARP genes Parp-9 and Parp-14 are developmentally and differentially regulated in mouse tissues
Antoinette Hakmé1, Aline Huber, Pascal Dollé
1Université Strasbourg 1, Institut Gilbert Laustriat, CNRS - UMR 7175, Département Intégrité du Génome, ESBS, Bld Sébastien Brant, BP 10413, Illkirch Cedex, France.
Abstract:
The macroPARPs Parp-9 and Parp-14 are macro domain containing poly(ADP-ribose) polymerases involved in transcriptional regulation in response to immunoregulatory cytokines. Their genes reside in the same locus (16B3), and the Parp-9 gene lies head-to-head and shares its promoter with the gene encoding its partner, Bbap. Here, we provide a detailed analysis of Parp-9, Parp-14, and Bbap expression during mouse development and adulthood. Parp-9 is developmentally regulated, and prominently expressed in the thymus and specific regions of the brain and gut. In adults, highest expression is maintained in the thymus and intestine. Parp-14 is more weakly expressed, mainly in the thymus during development and in adulthood. In addition, we show that Bbap is essentially coexpressed with Parp-9 during development and in adult mouse. However, the different levels of their transcripts detected in the developing brain and gut suggest that Bbap and Parp-9 display both common and independent tissue-specific regulations.
Insights
Poly(ADP-ribose) polymerases (PARPs) Parp-9 and Bbap show coordinated expression during mouse development and adulthood, particularly in the thymus and intestine. However, distinct transcript levels in the brain and gut indicate independent regulatory mechanisms.
Area of Science:
- Molecular Biology
- Genetics
- Immunology
Background:
- Macro domain-containing poly(ADP-ribose) polymerases (PARPs), specifically Parp-9 and Parp-14, are implicated in transcriptional regulation.
- Parp-9 and its interacting partner, Bbap, share a promoter and are located at the same genetic locus (16B3).
Purpose of the Study:
- To analyze the expression patterns of Parp-9, Parp-14, and Bbap throughout mouse development and in adulthood.
- To investigate the co-expression and potential independent regulation of Parp-9 and Bbap.
Main Methods:
- Quantitative analysis of gene expression during mouse development and in adult tissues.
- Comparison of expression profiles across different tissues, including the thymus, brain, and gut.
Main Results:
- Parp-9 exhibits developmental regulation, with prominent expression in the thymus, brain, and gut, maintained in the thymus and intestine in adults.
- Bbap is largely coexpressed with Parp-9 during both developmental and adult stages.
- Parp-14 shows weaker expression, primarily in the thymus.
- Differential transcript levels of Bbap and Parp-9 in the developing brain and gut suggest tissue-specific regulatory differences.
Conclusions:
- Parp-9 and Bbap display both shared and distinct tissue-specific expression patterns, indicating complex regulatory control.
- The findings provide insights into the developmental roles and regulation of these macroPARPs in mammals.
