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DNA distribution pattern in colorectal carcinoma. A methodologic study comparing two different biopsy techniques.

M Koha1, B Wikström, B Brismar

  • 1Department of Surgery, Huddinge University Hospital, Sweden.

Analytical and Quantitative Cytology and Histology
|December 1, 1991
PubMed
Summary
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Touch imprints of punch biopsies offer a reliable method for evaluating DNA patterns in colorectal tumors, showing high concordance with fine needle aspiration methods. This supports accurate DNA ploidy assessment for improved colorectal cancer diagnosis.

Area of Science:

  • Oncology
  • Molecular Pathology
  • Gastroenterology

Background:

  • Accurate DNA content analysis is crucial for diagnosing and classifying colorectal tumors.
  • Comparing different biopsy techniques for DNA ploidy assessment is essential for clinical utility.

Purpose of the Study:

  • To compare the reliability of fine needle aspiration (FNA) and touch imprints of punch (IMP) biopsies for DNA content measurement in colorectal tumors.
  • To assess the concordance of DNA ploidy patterns obtained by FNA and IMP methods.

Main Methods:

  • Single cell microspectrophotometry was used to measure DNA content.
  • 59 samples from 20 colorectal adenocarcinomas and 5 adenomas were analyzed.
  • DNA ploidy patterns were compared between FNA and IMP specimens.

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Main Results:

  • Specimens from FNA and IMP showed similar DNA ploidy patterns in 78% of samples (46/59).
  • Considering the most aggressive DNA pattern, concordance between FNA and IMP reached 88% (22/25 patients).
  • Discrepancies were observed in a minority of samples, with some aneuploid in one method and non-aneuploid in the other.

Conclusions:

  • Touch imprints of punch biopsies provide a reliable method for DNA pattern evaluation in colorectal tumors.
  • IMP method demonstrates high concordance with FNA for DNA ploidy assessment.
  • This technique supports accurate diagnostic and prognostic evaluations of colorectal neoplasms.