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[Selection of the microorganism for dioscin hydrolyze]
Yu-ting Zhao1, Zeng-lai Xu, Chuan-chao Dai
1College of Life Science, Nanjing Normal University, Nanjing 210046, China.
Objective:
To select the microorganism which can hydrolyze dioscin to diosgenin.
Methods:
The microorganism were selected from the surface of rhizome, rhizosphere soil, the inside of the leaves and rhizome of Dioscorea zingiberensis C. H. Wright. Diosgenin was identified by thin-layer chromatography and HPLC.
Results:
The microorganism which could hydrolyze dioscin from the experiment were identified as Aspergillus sp and Alternaria sp. Characteristics of enzymes production and fermentation technology of Aspergillus No. 1 were also studied primarily.
Conclusion:
The Aspergillus strain No. 1 can secret enzyme to hydrolyze dioscin into diosgenin effectively.
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