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Updated: Jul 9, 2026
Determination of the Procoagulant Activity of Extracellular Vesicle (EV) Using EV-Activated Clotting Time (EV-ACT)
Published on: August 4, 2023
[Study of anticoagulant activity of ethanol extracts from leech in vitro]
Guang-jun Feng1, Zheng-guang Zhu, Chuan-lin Yu
1Institute of Pharmaceutical Science, College of Pharmaceutical Science, Southern Medical University, Guangzhou 510515, China.
Objective:
To study the anticoagulant activity of different portions of leech ethanol extracts (LEEs).
Methods:
Anticoagulant activity was determined by measuring prothrombin time(PT), thrombin time (TT), activated partial throboplstin time (APTT) and fibrinogen coagulation time (FCT).
Results:
PT, TT, APTT and FCT were remarkably prolonged by ethyl acetate portion of LEEs. Portions of petroleum ethrer, n-butanol and water extracted from LEEs was much weaker on anticoagulant activity.
Conclusion:
The anticoagulant effect of ethyl acetate extract portion of LEEs is the strongest among the four portions, and this results may be from its inhibitory effect on thrombin-catalyzed fibrinogen hydrolysis.
