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Updated: Jul 9, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Study on purification of total flavonoids from Rosa laevigata with macroporous resin column chromatography]
Nai-fu Chen1, Ke Chen, Li Zhang
1Department of Chemistry and Life Science, West of Anhui University, Liuan 237012, China.
Objective:
To Study the technologic conditions of purifying total Rosa laevigata by AB-8 pocket.
Methods:
Extracting flavonoids from Rosa laevigata with 70% alcohol, the thick flavonoid powder were obtained and the content of pure flavonoids in it could be determinated using spectrophotometry. Taking the adsorption of the AB-8 pocket to Rosa flavonoids as the research object, the factors that affected the AB-8 resin static absorption and desorption were studied preliminarily.
Results:
The result showed that the effect was perfect when the flavononid concentration in the liquid was 0.3 mg/ml, the loading amount was 120 mg with the adsorption-power 1 ml/ min; And the volume of 70% alcohol was eluant 5 BV with desorption-power 1 ml/min. The pure flavonoid content in thick flavonoid powder was 25.2% and it reached 42.2% after being purified by the AB-8 pocket adsorption resin.
Conclusion:
It proved AB-8 pocket adsorption resin have a better effect on purification of the flavononids of Rosa laevigata.
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