Related Experiment Video
Updated: Jul 9, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
Identification of two E-boxes that negatively modulate the activity of MyoD on the alpha-sarcoglycan core promoter
Paul Delgado-Olguín1, J Manuel Hernández-Hernández, Fabio Salamanca
1Unidad de Investigación Médica en Genética Humana, Hospital de Pediatría, Centro Médico Nacional Siglo XXI-IMSS, Col. Doctores, Delegación Cuauhtémoc, México DF 06725, México.
Abstract:
The alpha-SG promoter is composed of a plethora of cis-regulatory elements, whose individual contribution to alpha-SG gene expression modulation remains unknown. We have identified a negative regulatory element in the alpha-SG distal promoter including two conserved E-boxes (E1 and E2), which interact with MyoD. We found that E1 and E2 negatively modulate the transactivation potential of MyoD on the alpha-SG core promoter. Moreover, such negative effect is mainly mediated by E2, which is surrounded by conserved nucleotides conferring MyoD binding capacity. Our results suggest that modulation of MyoD activity by E1, and particularly E2, contributes to the negative regulation of alpha-SG gene expression during myogenic differentiation.
Related Concept Videos
Master Transcription Regulators
Master Transcription Regulators
Eukaryotic Transcription Activators
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Co-activators and Co-repressors
Co-activators and Co-repressors
Cell Specific Gene Expression

