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An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
A rapid and validated HPLC method to quantify racecadotril metabolite, thiorphan, in human plasma using solid-phase
Fan Xu1, Lingli Yang, Guili Xu
1Kunming General Hospital of Chengdu Military Region, Kunming, Yunnan 650032, China. xu_fan@126.com
Summary
A new HPLC method accurately quantifies thiorphan in human plasma using UV detection and solid-phase extraction. This validated assay is crucial for pharmacokinetic studies, offering a simple and economical approach.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
Background:
- Accurate quantification of drugs in biological matrices is essential for pharmacokinetic studies.
- Thiorphan is an important pharmaceutical agent requiring reliable analytical methods for its determination.
Purpose of the Study:
- To develop and validate a high-performance liquid chromatography (HPLC) method for determining thiorphan concentrations in human plasma.
- To establish a robust and sensitive analytical technique suitable for pharmacokinetic investigations.
Main Methods:
- High-performance liquid chromatography (HPLC) with UV detection at 210 nm.
- Solid-phase extraction (SPE) for plasma sample pre-treatment using Oasis HLB cartridges.
- Separation on a Waters Sunfire C18 reversed-phase column with a mobile phase of phosphate buffer and acetonitrile (74:26, v/v).
Main Results:
- The method demonstrated excellent linearity (r > 0.9998) over the range of 0.05-4 microg/mL.
- High extraction recovery (93.5-98.2%) and a low limit of quantification (LOQ) of 0.05 microg/mL were achieved.
- The method exhibited good accuracy (92.7-99.6%) and precision (CV 2.2-8.4% within-batch, 4.1-8.1% between-batch).
Conclusions:
- The developed HPLC-UV method is simple, economical, specific, and validated for thiorphan determination in human plasma.
- This method is suitable for application in pharmacokinetic studies.
- The assay provides reliable quantification essential for understanding thiorphan's behavior in vivo.
