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Updated: Jul 9, 2026

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Published on: January 12, 2015

Cardiomyocytes from human and mouse embryonic stem cells.

Christine Mummery¹, Marcel A G van der Heyden, Teun P de Boer

¹Hubrecht Laboratory, Utrecht, the Netherlands.

Methods in Molecular Medicine

|December 19, 2007

Summary

Human and mouse embryonic stem cells can become cardiomyocytes, aiding heart development research and potential cell therapies. Protocols vary by cell line and species, impacting cell characterization and transplantation readiness.

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Area of Science:

Stem cell biology
Cardiovascular research
Developmental biology

Background:

Embryonic stem (ES) cells offer potential for studying heart development.
ES cell-derived cardiomyocytes are of interest for drug response studies and regenerative medicine.
Species-specific and cell-line-specific factors influence ES cell differentiation into cardiomyocytes.

Purpose of the Study:

To describe methods for generating and characterizing cardiomyocytes from human and mouse ES cells.
To detail techniques for dissociating cardiomyocytes into single-cell suspensions.
To highlight the utility of these methods for cell characterization and transplantation.

Main Methods:

Generation of cardiomyocytes from human and mouse embryonic stem cells.

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Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Published on: June 28, 2013

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Last Updated: Jul 9, 2026

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Published on: January 12, 2015

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells

Published on: August 9, 2017

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Generation of Human Cardiomyocytes: A Differentiation Protocol from Feeder-free Human Induced Pluripotent Stem Cells

Published on: June 28, 2013

Characterization of differentiated cardiomyocytes.

Dissociation of cardiomyocytes into single-cell suspensions.

Antibody staining and electrophysiological measurements for cell characterization.

Main Results:

Established protocols for generating cardiomyocytes from both human and mouse ES cells.
Developed methods for single-cell suspension preparation.
Demonstrated utility of methods for cell analysis and potential therapeutic applications.

Conclusions:

Embryonic stem cells provide a valuable model for studying cardiac development and cardiomyocyte properties.
Effective differentiation and dissociation protocols are crucial for research and therapeutic applications.
These methods facilitate detailed cell characterization and preparation for cardiac repair strategies.