Related Experiment Video
Updated: Jul 9, 2026
Fabrication And Characterization Of Photonic Crystal Slow Light Waveguides And Cavities
Published on: November 30, 2012
Collinear type II second-harmonic-generation frequency-resolved optical gating for use with high-numerical-aperture
D N Fittinghoff1, J A Squier, C P Barty
1Institute for Nonlinear Science, University of California, San Diego, La Jolla, California 92093-0339, USA.
Abstract:
Ultrashort-pulse lasers are now commonly used for multiphoton microscopy, and optimizing the performance of such systems requires careful characterization of the pulses at the tight focus of the microscope objective. We solve this problem by use of a collinear geometry in frequency-resolved optical gating that uses type II second-harmonic generation and that allows the full N.A. of the microscope objective to be used. We then demonstrate the technique by measuring the intensity and the phase of a 22-fs pulse focused by a 20x, 0.4-N.A. air objective.
Related Concept Videos
01:26Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
01:18Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...