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Updated: Jul 9, 2026
08:35
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
Published on: June 24, 2021
Identification, classification, and expression of RAGE gene splice variants.
Barry I Hudson1, Angela M Carter, Evis Harja
1Division of Surgical Science, Department of Surgery, College of Physicians and Surgeons, Columbia University, 630 W. 168th St., PS 17-401, New York, New York 10032, USA. bh2021@columbia.edu
Summary
Researchers identified diverse human splice variants of the receptor for advanced glycation end-products (RAGE). These variants impact RAGE function and regulation, offering new insights into disease and normal biological processes.
Area of Science:
- Molecular Biology
- Genetics
- Immunology
Background:
- The receptor for advanced glycation end-products (RAGE) is a key mediator in various diseases.
- Understanding RAGE regulation is crucial for disease pathogenesis and homeostasis.
Purpose of the Study:
- To characterize and identify human RAGE splice variants.
- To investigate the functional implications of these variants.
Main Methods:
- Analysis of RAGE cDNA from human tissues and cells.
- Classification of splice variants according to HGNC.
- Nonsense-mediated mRNA decay (NMD) pathway analysis.
- Expression analysis of RAGE variants.
Main Results:
- A wide range of RAGE splice variants were identified, altering the protein coding region.
- Variants affect the ligand-binding domain or result in loss of transmembrane/cytosolic domains.
- Approximately 50% of variants are targeted for NMD.
- RAGE_v1 was identified as the primary secreted soluble isoform.
Conclusions:
- The RAGE gene exhibits significant biological diversity through functional splice variants.
- These findings enhance understanding of RAGE in normal physiology and disease states.
- Identification of RAGE splice variants provides novel targets for therapeutic intervention.
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