Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 9, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Cell-specific internalization study of an aptamer from whole cell selection.

Zeyu Xiao¹, Dihua Shangguan, Zehui Cao

¹Center for Research at the Bio/Nano Interface, Department of Chemistry, Shands Cancer Center and UF Genetics Institute, McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200, USA.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|December 20, 2007

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neutrophil extracellular trap (NET)-related index as an indicator of periprosthetic joint infection.

Journal of bone and joint infection·2026

Same author

Construction of Agonistic Bivalent Double-Stranded Aptamers Targeting c-MET.

Angewandte Chemie (International ed. in English)·2026

Same author

Self-oxidation and proximity crosslinking of G-quadruplex in G-quadruplex /hemin peroxidase system.

Talanta·2026

Same author

Aptamers Generated by Multi-Strategy Selection Target FcγRI and Block IgG Fc-FcγRI Interaction.

Analytical chemistry·2025

Same author

Detection and Verification of Intestinal and Placental Alkaline Phosphatase Heterodimer in Extracellular Fluid.

ACS applied bio materials·2025

Same author

Selection and identification of a novel DNA aptamer for recognizing colorectal cancer stem cells.

Bioorganic chemistry·2025

A new DNA aptamer, sgc8, is efficiently internalized by lymphoblastic leukemia cells. This aptamer shows promise for targeted intracellular delivery without observed cytotoxicity, opening new avenues for aptamer applications.

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Nucleic acid aptamers are valuable for molecular recognition but face challenges in cellular internalization for functional studies and intracellular delivery.
Limited availability of cell-specific aptamers and understanding of their in vivo functions hinder their broader application.
Most aptamers require external assistance for cellular uptake, unlike small molecules.

Purpose of the Study:

To investigate the cellular internalization and intracellular fate of a newly developed, cell-specific DNA aptamer, sgc8.
To evaluate the potential of sgc8 for targeted intracellular delivery into specific cell types.
To compare the internalization kinetics of sgc8 with an antibody targeting the same cell surface protein.

Main Methods:

More Related Videos

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Related Experiment Videos

Last Updated: Jul 9, 2026

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

Published on: September 13, 2022

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Development of Cell-type specific anti-HIV gp120 aptamers for siRNA delivery

Published on: June 23, 2011

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses

Published on: September 7, 2022

Selection of a cell-specific DNA aptamer (sgc8) using a novel cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process.
Studying the cellular internalization and subcellular localization of sgc8 in lymphoblastic leukemia cells.
Comparing the internalization of sgc8 with an antibody against PTK7 (Human protein tyrosine kinase-7) on living cells.
Assessing the cytotoxicity of sgc8 on cell viability.

Main Results:

The DNA aptamer sgc8 was efficiently and specifically internalized into lymphoblastic leukemia cells.
Internalized sgc8 aptamers were primarily localized within endosomes.
sgc8 demonstrated comparable internalization kinetics to an anti-PTK7 antibody and exhibited no cytotoxicity.
The cell-SELEX process successfully generated a cell-specific aptamer with potential for intracellular delivery.

Conclusions:

The cell-specific DNA aptamer sgc8 is efficiently internalized into target cells and resides in endosomes.
sgc8 is a promising candidate for cell-type specific intracellular delivery applications.
This study highlights the potential of aptamers selected via cell-SELEX for advanced biological and therapeutic applications.