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Selection of Aptamers for Amyloid β-Protein, the Causative Agent of Alzheimer's Disease

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Aptamers Generated by Multi-Strategy Selection Target FcγRI and Block IgG Fc-FcγRI Interaction.

Jing Sheng^1,2, Tingrun Cui^3,4, Yang Xu¹

¹Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Bio-systems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Analytical Chemistry

|December 8, 2025

Summary

This summary is machine-generated.

Researchers developed high-affinity aptamers targeting the Fc gamma receptor I (FcγRI). These aptamers effectively block IgG binding, showing potential for immune regulation and diagnostics.

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Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics DCAF

Targeted Antibody Blocking by a Dual-Functional Conjugate of Antigenic Peptide and Fc-III Mimetics DCAF

Published on: September 17, 2019

Area of Science:

Immunology
Biotechnology
Molecular Biology

Background:

Fc gamma receptor I (FcγRI) is crucial for immune responses, mediating phagocytosis and cytokine secretion.
Targeting FcγRI with high-affinity ligands offers potential for immune modulation and diagnostics.

Purpose of the Study:

To isolate and engineer high-affinity aptamers specific for human FcγRI.
To evaluate the aptamers' ability to block FcγRI-IgG Fc interactions and their diagnostic applications.

Main Methods:

Integrated Capture-SELEX, PAGE-SELEX, and Cell-SELEX for aptamer selection.
Employed truncation and site-directed mutagenesis for aptamer optimization.
Assessed aptamer affinity and inhibitory capacity using binding assays.

Main Results:

Aptamers with high specificity for human FcγRI were successfully isolated.
Engineered aptamers exhibited enhanced binding affinity (Kd: 0.98 to 0.15 nM) and potent inhibition (IC50: 3.55 to 0.88 nM).
Optimized aptamers were applied in analyzing FcγRI-expressing cells, detecting upregulation, and enriching target cells.

Conclusions:

Developed aptamers effectively bind and inhibit FcγRI-IgG Fc interactions.
Optimized aptamers demonstrate significant potential for immunological research and clinical diagnostics.