Related Experiment Video
Updated: Jul 9, 2026
13:56
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Published on: July 18, 2013
AAV-2 Rep78 and HPV-16 E1 interact in vitro, modulating their ATPase activity
Sarmistha Bandyopadhyay1, Kevin D Raney, Yong Liu
1Department of Internal Medicine, Gene Therapy Program, University of Arkansas for Medical Sciences, 4301 West Markham Street, Little Rock, Arkansas 72205, USA.
Biochemistry
|December 21, 2007
Summary
Human papillomavirus (HPV) E1 protein interacts with adeno-associated virus (AAV) Rep78 protein. This interaction modulates their biochemical functions, offering insights into AAV DNA replication mechanisms.
Area of Science:
- Virology
- Molecular Biology
- Biochemistry
Background:
- Adeno-associated virus (AAV) requires helper viruses for efficient DNA replication.
- Human papillomavirus (HPV) can function as an AAV helper virus.
- The HPV-16 E1 protein is a key helper gene for AAV-2 DNA replication and transcription.
Purpose of the Study:
- To investigate the physical interaction between AAV Rep78 and HPV E1 proteins.
- To determine if the homology between Rep78 and E1 suggests a functional interaction.
- To explore the impact of this interaction on their respective biochemical activities.
Main Methods:
- Purification of full-length GST-E1 and MBP-Rep78 proteins from bacteria.
- Utilized five biochemical assays: pull-down, coimmunoprecipitation, ELISA, chemical cross-linking, and ATPase activity assays.
- Assessed the effect of combined protein presence on ATPase activity.
Main Results:
- Multiple assays provided consistent evidence of a physical interaction between Rep78 and E1.
- The ATPase activity of the proteins was decreased when both were present together.
- The interaction suggests modulation of individual protein functions.
Conclusions:
- AAV Rep78 and HPV E1 proteins physically interact.
- This interaction influences their biochemical functions, particularly ATPase activity.
- Provides foundational insights into the mechanism of HPV E1's helper function in AAV DNA replication.
Related Concept Videos
01:19Inhibitors of Virion Maturation and Assembly
As part of their replication cycle, certain viruses synthesize long precursor proteins called polyproteins within infected host cells. In human immunodeficiency virus (HIV), two major polyproteins are produced: Gag and Gag-Pol. The Gag polyprotein supplies the structural components of the virus, while Gag-Pol includes essential viral enzymes such as reverse transcriptase, integrase, and protease. After synthesis, these polyproteins move to the host cell membrane, where they assemble into an...
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
01:51Mechanisms of Retrovirus-induced Cancers
Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
01:16In vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:16In-vitro Mutagenesis
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
01:25Inhibitors Of Virion Release
Viral replication and dissemination rely on efficient mechanisms for host cell entry, genome replication, assembly, and release. Influenza viruses, such as types A and B, are negative-sense single-stranded RNA viruses with a segmented genome, that depend on two critical surface glycoproteins to carry out these processes: hemagglutinin (HA) and neuraminidase (NA). HA initiates infection by binding to sialic acid residues on the surface of host epithelial cells, facilitating receptor-mediated...